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. 1968 Feb 23;159(3817):884-5.
doi: 10.1126/science.159.3817.884.

Antigen binding to cells: determination by enzymic fluorogenic group hydrolysis

E E Sercarz, F Modabber

Antigen binding to cells: determination by enzymic fluorogenic group hydrolysis

E E Sercarz et al. Science. .

Abstract

A sensitive method for detecting cells containing antibody to beta-galactosidase has been devised. The enzyme attached to the cells containing antibody can hydrolyze a fluorogenic substrate and yield fluorescent products which are measured microphotofluorometrically. This method of detecting a few molecules of antibody is applicable to other enzyme antigen systems.

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