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. 1968 Feb;106(3):601-7.
doi: 10.1042/bj1060601.

Studies on extracellular ribonucleases of Ustilago sphaerogena. Purification and properties

T Arima, T Uchida, F Egami

Studies on extracellular ribonucleases of Ustilago sphaerogena. Purification and properties

T Arima et al. Biochem J. 1968 Feb.

Abstract

1. Four ribonucleases were isolated from culture media of Ustilago sphaerogena. They were designated ribonucleases U(1), U(2), U(3) and U(4). 2. They were purified about 1600-, 3700-, 1100- and 16-fold respectively. 3. It was shown by gel filtration that ribonucleases U(1), U(2) and U(3) have molecular weights about 10000 like ribonuclease T(1), and that ribonuclease U(4) is much larger. 4. Ribonucleases U(1), U(2) and U(3) are thermostable, but ribonuclease U(4) is not. 5. The pH optimum of ribonucleases U(1) and U(4) is pH8.0-8.5, and that of ribonucleases U(2) and U(3) is pH4.5.

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