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. 1968 Apr;107(3):427-31.
doi: 10.1042/bj1070427.

Chromatography of oxidized and reduced cytochrome c on carboxymethylcellulose

H B Dixon, C M Thompson

Chromatography of oxidized and reduced cytochrome c on carboxymethylcellulose

H B Dixon et al. Biochem J. 1968 Apr.

Abstract

1. Cytochrome c was isolated from horse heart by a chromatographic method. 2. Oxidized and reduced cytochrome c were chromatographed on CM-cellulose that was in equilibrium with several buffer systems of constant composition at pH values of 8.4, 6.75 and 4.9. 3. Separation was better at the higher pH values; the oxidized form was retarded more than twice as much as the reduced form, though they differed by only a single charge. 4. Self-competition between cytochrome molecules is suggested to account for the peak distortion observed at high loads (above 20mum protein concentration).

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