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. 1968 Mar;95(3):1118-23.
doi: 10.1128/jb.95.3.1118-1123.1968.

Hydrogen-oxidizing methane bacteria. I. Cultivation and methanogenesis

M P Bryant, B C McBride, R S Wolfe

Hydrogen-oxidizing methane bacteria. I. Cultivation and methanogenesis

M P Bryant et al. J Bacteriol. 1968 Mar.

Abstract

A method for the mass culture of hydrogen-oxidizing methane bacteria has been developed; yields of 50 to 60 g (wet weight) of cells per 12 to 14 liters of culture medium were obtained. The methanogenic organism from the culture of Methanobacillus omelianskii was grown in a complex medium which was aerated with a gas mixture of hydrogen and carbon dioxide. Extracts prepared from hydrogen-grown cells formed methane from methyl cobalamin, 5-methyl tetrahydrofolate, serine, pyruvate, and carbon dioxide; these substrates have been shown to be precursors of methane in extracts of the ethyl alcohol-grown culture of M. omelianskii.

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References

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