doi: 10.1126/science.161.3841.586.
Bioluminescence: pH activity profiles of related luciferase fractions
- PMID: 5663301
- DOI: 10.1126/science.161.3841.586
Item in Clipboard
Bioluminescence: pH activity profiles of related luciferase fractions
Science.
.
Abstract
The 27, 000g supernatant from crude extracts of the dinoflagellate Gonyaulax can be fractionated into two components having luciferase activity that differ both in molecular weight (35, 000 and 150, 000) and in pH activity profile. The smaller component has activity over a broad range from pH 5 to 9, while the larger one is active only in the acid region. This clarifies the previous ambiguity in the literature regarding the optimum pH for the assay of luciferase.
Similar articles
-
Dinoflagellate bioluminescence: a comparative study of invitro components.J Cell Physiol. 1976 Jan;87(1):123-34. doi: 10.1002/jcp.1040870115. J Cell Physiol. 1976. PMID: 1400
-
An active proteolytic fragment of Gonyaulax luciferase.Biochemistry. 1974 Jul 2;13(14):2871-7. doi: 10.1021/bi00711a015. Biochemistry. 1974. PMID: 4407615 No abstract available.
-
A substrate-binding protein in the Gonyaulax bioluminescence reaction.Arch Biochem Biophys. 1971 Jan;142(1):310-21. doi: 10.1016/0003-9861(71)90289-x. Arch Biochem Biophys. 1971. PMID: 5545485 No abstract available.
-
The structural origin and biological function of pH-sensitivity in firefly luciferases.Photochem Photobiol Sci. 2008 Feb;7(2):159-69. doi: 10.1039/b714392c. Epub 2008 Jan 24. Photochem Photobiol Sci. 2008. PMID: 18264583 Review.
-
Evolution of beetle bioluminescence: the origin of beetle luciferin.Luminescence. 2004 Jan-Feb;19(1):8-20. doi: 10.1002/bio.749. Luminescence. 2004. PMID: 14981641 Review.
Cited by
-
Transcendent Aspects of Proton Channels.Annu Rev Physiol. 2024 Feb 12;86:357-377. doi: 10.1146/annurev-physiol-042222-023242. Epub 2023 Nov 6. Annu Rev Physiol. 2024. PMID: 37931166 Free PMC article. Review.
-
Bioluminescence: mechanism and mode of control of scintillon activity.Proc Natl Acad Sci U S A. 1972 Mar;69(3):690-3. doi: 10.1073/pnas.69.3.690. Proc Natl Acad Sci U S A. 1972. PMID: 4501583 Free PMC article.
-
The Voltage-Gated Proton Channel: A Riddle, Wrapped in a Mystery, inside an Enigma.Biochemistry. 2015 Jun 2;54(21):3250-68. doi: 10.1021/acs.biochem.5b00353. Epub 2015 May 20. Biochemistry. 2015. PMID: 25964989 Free PMC article. Review.
-
Solubilization of molecular elements of scintillons: bioluminescent particles from dinoflagellates.Mol Cell Biochem. 1974 Apr 15;3(2):81-91. doi: 10.1007/BF01659180. Mol Cell Biochem. 1974. PMID: 4832397 No abstract available.
-
Two different domains of the luciferase gene in the heterotrophic dinoflagellate Noctiluca scintillans occur as two separate genes in photosynthetic species.Proc Natl Acad Sci U S A. 2007 Jan 16;104(3):696-701. doi: 10.1073/pnas.0607816103. Epub 2006 Nov 27. Proc Natl Acad Sci U S A. 2007. PMID: 17130452 Free PMC article.
