doi: 10.1042/bj1080861.
The location of the active-site histidine residue in the primary sequence of papain
- PMID: 5673531
- PMCID: PMC1198893
- DOI: 10.1042/bj1080861
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The location of the active-site histidine residue in the primary sequence of papain
Biochem J.
1968 Aug.
Abstract
Papain that had been irreversibly inhibited with 1,3-dibromo[2-(14)C]acetone was reduced with sodium borohydride and carboxymethylated with iodoacetic acid. After digestion with trypsin and alpha-chymotrypsin the radioactive peptides were purified chromatographically. Their amino acid composition indicated that cysteine-25 and histidine-106 were cross-linked. Since cysteine-25 is known to be the active-site cysteine residue, histidine-106 must be the active-site histidine residue.
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