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. 1968 Aug;108(5):861-6.
doi: 10.1042/bj1080861.

The location of the active-site histidine residue in the primary sequence of papain

S S Husain, G Lowe

The location of the active-site histidine residue in the primary sequence of papain

S S Husain et al. Biochem J. 1968 Aug.

Abstract

Papain that had been irreversibly inhibited with 1,3-dibromo[2-(14)C]acetone was reduced with sodium borohydride and carboxymethylated with iodoacetic acid. After digestion with trypsin and alpha-chymotrypsin the radioactive peptides were purified chromatographically. Their amino acid composition indicated that cysteine-25 and histidine-106 were cross-linked. Since cysteine-25 is known to be the active-site cysteine residue, histidine-106 must be the active-site histidine residue.

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References

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