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. 1968 Aug;16(8):1245-50.
doi: 10.1128/am.16.8.1245-1250.1968.

Production of highly labeled adenoviruses

J P Burnett, A O Summers, J A Harrington, A C Dwyer

Production of highly labeled adenoviruses

J P Burnett et al. Appl Microbiol. 1968 Aug.

Abstract

A method is described for increasing the incorporation of radioactive thymidine into adenovirus deoxyribonucleic acid by the use of amethopterin. In addition, a modified procedure is presented for the preparation of highly purified adenoviruses. This procedure, which employs enzymatic digestion of cellular debris, obviates the necessity for fluorocarbon treatment of crude virus suspensions, and routinely provides excellent recovery of virus.

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References

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