Plasma protein handling in the rat kidney: micropuncture experiments in the acute heterologous phase of anti-GBM-nephritis

Abstract

Glomerular filtration and tubular uptake of plasma proteins have been studied in the rat using micropuncture techniques. 1. Under control conditions the glomerular capillary wall is an effective barrier, only 7.6 microgram/min x 100 g BW albumin have been measured as filtered load. Four to twelve hours after i.v. injection of anti-glomerular-basement membrane serum (anti-GBM-serum sieving coefficient phi and filtered load increased in a dose-dependent manner (phi albumin in controls = 0.27 x 10(-3), after injection of 0.5 ml Antiserum phi = 0.28 x 10(-3) and 1.0 ml Antiserum phi = 2.32 x 10(-3)). 2. The tubular reabsorption capacity is almost reached under control conditions and amounts to 5.6--10.7 microgram/min x 100 g BW for albumin. Only reduced GFR (0.36 +/- 0.07 ml/min x 100 g BW) and reduced tubular flow lead to increased tubular uptake under overload conditions (10.7 vs. 99.0 microgram albumin/min x 100 g BW). 3. Tubular reabsorption of so-called high-molecular-weight proteins seems to be a nonselective mechanism. The ratio Alb/Alb + Glob (89.9--93.1%) did not differ significantly at the individual puncture sites and in the final urine.