Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1968 Nov;39(2):392-403.
doi: 10.1083/jcb.39.2.392.

Aggregation and transformation of rat lymphocytes on rat embryo monolayers

H Ginsburg, D Lagunoff

Aggregation and transformation of rat lymphocytes on rat embryo monolayers

H Ginsburg et al. J Cell Biol. 1968 Nov.

Abstract

Lymphocytes from Sprague-Dawley rats have been cultured on monolayers of embryo-derived fibroblasts from the same outbred strain. Under these conditions the lymphocytes form aggregates, and transformation of small lymphocytes to lymphoid blast cells occurs within these aggregates. Transformation is characterized cytologically by enlargement of the nucleus, dispersion of nuclear chromatin, and the appearance of a prominent nucleolus. The principal cytoplasmic changes are an increase in cytoplasmic volume, a marked increase in number of ribosomes, and a clustering of ribosomes. These changes parallel those seen in the transformation of lymphocytes caused by a variety of treatments. One apparent difference is the paucity of lysosomes and lipid inclusions in the lymphocytes that transform on the monolayer.

PubMed Disclaimer

References

    1. Science. 1965 Sep 17;149(3690):1385-7 - PubMed
    1. Lab Invest. 1965 Oct;14(10):1736-43 - PubMed
    1. Lab Invest. 1966 Feb;15(2):455-63 - PubMed
    1. J Exp Med. 1966 Nov 1;124(5):873-84 - PubMed
    1. J Immunol. 1967 Jan;98(1):17-30 - PubMed