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. 1968 Sep;109(3):389-98.
doi: 10.1042/bj1090389.

Choline acetyltransferase binding to and release from membranes

F Fonnum

Choline acetyltransferase binding to and release from membranes

F Fonnum. Biochem J. 1968 Sep.

Abstract

1. The binding of non-occluded choline acetyltransferase to synaptosome membranes is a reversible process that is primarily dependent on the pH and ionic strength of the suspending medium. 2. The distribution of soluble enzyme bound to synaptosome membranes was studied by density-gradient centrifuging. 3. Choline acetyltransferase shows enzyme activity both in the free and in the membrane-bound form. 4. Varying the temperature or prolonged hypo-osmotic treatment does not release the membrane-bound enzyme. 5. The release of choline acetyltransferase from membranes by different anions, thiols, adenosine nucleotides and enzyme substrates was studied.

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