doi: 10.1093/nar/5.11.4155.
Partial purification of transcriptionally active nucleosomes from trout testis cells
- PMID: 569293
- PMCID: PMC342740
- DOI: 10.1093/nar/5.11.4155
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Partial purification of transcriptionally active nucleosomes from trout testis cells
Nucleic Acids Res.
1978 Nov.
Free PMC article
Abstract
Mononucleosomes (MN1) enriched in structural non-histone proteins and transcribed DNA sequences were obtained by limited digestion of trout testis nuclei with micrococcal nuclease followed by selective solubilization in 0.1 M NaCl. These monosomes consist of the four inner histones plus stoichiometric amounts of the non-histone protein H6, of the HMG group, complexed with 140 base pairs of DNA. Hybridization experiments indicate that MN1 DNA is enriched in sequences complementary to cytoplasmic polyadenylated RNA.
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