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. 1978 Nov;5(11):4155-63.
doi: 10.1093/nar/5.11.4155.

Partial purification of transcriptionally active nucleosomes from trout testis cells

B Levy, G H Dixon
Free PMC article

Partial purification of transcriptionally active nucleosomes from trout testis cells

B Levy et al. Nucleic Acids Res. 1978 Nov.
Free PMC article

Abstract

Mononucleosomes (MN1) enriched in structural non-histone proteins and transcribed DNA sequences were obtained by limited digestion of trout testis nuclei with micrococcal nuclease followed by selective solubilization in 0.1 M NaCl. These monosomes consist of the four inner histones plus stoichiometric amounts of the non-histone protein H6, of the HMG group, complexed with 140 base pairs of DNA. Hybridization experiments indicate that MN1 DNA is enriched in sequences complementary to cytoplasmic polyadenylated RNA.

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