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. 1968 Jul;3(6):571-84.

Lymphocyte transformation in vitro. I. Tissue culture conditions and quantitative measurements

P T Schellekens, V P Eijsvoogel

Lymphocyte transformation in vitro. I. Tissue culture conditions and quantitative measurements

P T Schellekens et al. Clin Exp Immunol. 1968 Jul.

Abstract

The quantitative evaluation of the reactivity in human PHA-stimulated lymphocyte cultures was studied. A technique for the measurement of DNA synthesis by means of thymidine-2-14C incorporation was standardized and its reproducibility determined. Using this method a number of variables in culture conditions were investigated. The results obtained are discussed.

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References

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