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. 1979 Feb 15;4(4):217-21.
doi: 10.1007/BF00777557.

Chicken ribosomal DNA: gene frequency and purification by R-loop hybridization

C Lehmann, M Warnhoff, W Knochel, D Lange, J Born, H Tiedemann

Chicken ribosomal DNA: gene frequency and purification by R-loop hybridization

C Lehmann et al. Mol Biol Rep. .

Abstract

The genes for ribosomal RNA of chicken are present in about 200--240 copies per haploid genome. The ribosomal genes were separated from the bulk of DNA by different methods. In CsCl-actinomycin D gradients the GC-rich ribosomal DNA is shifted to the lower density side of the bulk of DNA. After hybridization with iodinated ribosomal RNA under conditions where R-loops are formed ribosomal DNA has an increased density. The R-loops can be separated from the bulk of DNA in Cs2-SO4 gradients.

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