Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1979 Feb 15;4(4):217-21.
doi: 10.1007/BF00777557.

Chicken ribosomal DNA: gene frequency and purification by R-loop hybridization

C Lehmann, M Warnhoff, W Knochel, D Lange, J Born, H Tiedemann

Chicken ribosomal DNA: gene frequency and purification by R-loop hybridization

C Lehmann et al. Mol Biol Rep. .

Abstract

The genes for ribosomal RNA of chicken are present in about 200--240 copies per haploid genome. The ribosomal genes were separated from the bulk of DNA by different methods. In CsCl-actinomycin D gradients the GC-rich ribosomal DNA is shifted to the lower density side of the bulk of DNA. After hybridization with iodinated ribosomal RNA under conditions where R-loops are formed ribosomal DNA has an increased density. The R-loops can be separated from the bulk of DNA in Cs2-SO4 gradients.

PubMed Disclaimer

References

    1. Nucleic Acids Res. 1976 Sep;3(9):2303-8 - PubMed
    1. J Gen Physiol. 1951 Mar 20;34(4):451-62 - PubMed
    1. Wilhelm Roux Arch Entwickl Mech Org. 1972 Jun;171(2):160-169 - PubMed
    1. Proc Natl Acad Sci U S A. 1972 Mar;69(3):576-80 - PubMed
    1. Prog Nucleic Acid Res Mol Biol. 1971;11:351-89 - PubMed

LinkOut - more resources