Separation of ten reovirus genome segments by polyacrylamide gel electrophoresis
- PMID: 5723704
- PMCID: PMC375428
- DOI: 10.1128/JVI.2.10.986-991.1968
Separation of ten reovirus genome segments by polyacrylamide gel electrophoresis
Abstract
Double-stranded ribonucleic acid (RNA) extracted from purified reoviruses of all three serotypes and from type 3 virus-infected cells was analyzed by polyacrylamide gel electrophoresis. It was calculated that each RNA includes 10 segments: 3 large, 3 intermediate, and 4 small fragments corresponding to molecular weights of about 2.5, 1.4, and 0.8 x 10(6) daltons, respectively, or a total of 15 x 10(6) daltons.
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