Diurnal rhythm of pituitary-thyroid axis in male rats and the effect of adrenalectomy

Abstract

Levels of TSH, thyroxine (T4) and triiodothyronine (T3) were measured by radioimmunoassay in plasma (TSH, T4, T3) and pituitary (TSH) of 60-day old male Long-Evans rats. Definite diurnal rhythms were demonstrated in pituitary TSH, plasma TSH and T3 in intact rats, evidenced by the statistically significant differences between zenith and nadir for pituitary TSH, plasma TSH and T3. The zenith value of pituitary TSH and the nadir values of plasma TSH, T4 and T3 were observed at the same time (2400 h) as were the nadir value of pituitary TSH and the zenith values of plasma TSH, T4, and T3 (at 1200 h). Our results indicate that the rhythmicity of pituitary TSH content is a mirror image of that of plasma TSH. Adrenalectomy not only reduced plasma corticosterone levels to almost zero, but also decreased plasma T3 and T4 levels. In adrenalectomized rats, the absolute concentration of pituitary and plasma TSH increased at 1200 h and at 2400 h and, in both cases, the difference between values at 1200 h and at 2400 h persisted. The differences in plasma TSH, T3 and T4 between 1200 h and 2400 h were also observed in sham-adrenalectomized rats. These results suggest glucocorticoids do influence the pituitary-thyroid axis, but that the rhythmicity of the pituitary-thyroid axis does not seem to depend on the rhythmicity of glucocorticoid secretion.