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. 1979 Aug 10;6(11):3471-80.
doi: 10.1093/nar/6.11.3471.

Partial purification and characterization of the messenger RNA for cell fibronectin

J B Fagan, K M Yamada, B de Crombrugghe, I Pastan
Free PMC article

Partial purification and characterization of the messenger RNA for cell fibronectin

J B Fagan et al. Nucleic Acids Res. .
Free PMC article

Abstract

Fibronectin mRNA has been partially purified by guanidine extraction, oligo-(dT)-cellulose chromatography and sucrose density gradient centrifugation. We obtain a fraction which programs a wheat germ in vitro translation system to synthesize a polypeptide species which co-electrophoreses with fibronectin in SDS-polyacrylamide gels and which is immunoprecipitated with affinity purified fibronectin-specific IgG. Analysis of this RNA fraction by methyl mercury hydroxide-agarose gel electrophoresis reveals the presence of a band accounting for 30 percent to 50 percent of the ethidium bromide-staining material in the fraction. The RNA of this band has an estimated molecular weight of about 3 million daltons and is greatly reduced in the corresponding RNA fraction from RSV transformed CEF. This RNA has been tentatively identified as fibronectin mRNA.

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