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. 1979 Dec;140(3):1013-6.
doi: 10.1128/jb.140.3.1013-1016.1979.

Cobalamins and cobalamin-dependent enzymes in Candida utilis

J M Poston, B A Hemmings

Cobalamins and cobalamin-dependent enzymes in Candida utilis

J M Poston et al. J Bacteriol. 1979 Dec.

Abstract

Candida utilis has been shown to contain 4.7 pmol of cobalamin per g of wet cell paste. Purification of the cobalamin showed it to be a mixture of methylcobalamin and adenosylcobalamin. Two cobalamin-dependent enzyme systems have been found in the yeast: methylcobalamin-dependent methionine biosynthesis and leucine 2,3-aminomutase. The cobalamin extracted from the yeast is as effective as authentic adenosylcobalamin in stimulating leucine 2,3-aminomutase.

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