Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1979 Nov 15;184(2):427-30.
doi: 10.1042/bj1840427.

Cell-free conversion of delta-(L-alpha-aminoadipyl)-L-cysteinyl-D-valine into an antibiotic with the properties of isopenicillin N in Cephalosporium acremonium

T Konomi, S Herchen, J E Baldwin, M Yoshida, N A Hunt, A L Demain

Cell-free conversion of delta-(L-alpha-aminoadipyl)-L-cysteinyl-D-valine into an antibiotic with the properties of isopenicillin N in Cephalosporium acremonium

T Konomi et al. Biochem J. .

Abstract

Cell-free extracts of antibiotic-negative mutants of Cephalosporium acremonium converted delta-(L-alpha-aminoadipyl)-L-cysteinyl-D-valine (LLD-tripeptide) into an antibiotic that was destroyed by penicillinase. The enzymic activity of the extracts was destroyed by boiling, but was not inhibited by cycloheximide. LLL-Tripeptide was totally inactive as substrate. The product resembled isopenicillin N, but not penicillin N, in its antibacterial spectrum. We propose that isopenicillin N is the first product of cyclization of LLD-tripeptide.

PubMed Disclaimer

References

    1. Nature. 1963;198:383-4 - PubMed
    1. Biochem J. 1976 Sep 1;157(3):651-60 - PubMed
    1. Proc Natl Acad Sci U S A. 1978 Dec;75(12):6253-7 - PubMed
    1. Biochem J. 1979 Nov 15;184(2):421-6 - PubMed
    1. Biochem Biophys Res Commun. 1976 May 17;70(2):465-73 - PubMed

MeSH terms