Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1969 Jan;16(1):35-43.

Glutaraldehyde as a coupling reagent in passive haemagglutination

E Onkelinx, W Meuldermans, M Joniau, R Lontie

Glutaraldehyde as a coupling reagent in passive haemagglutination

E Onkelinx et al. Immunology. 1969 Jan.

Abstract

A sensitive passive haemagglutination technique is described depending on the attachment of proteins to erythrocytes with glutaraldehyde. Various factors that play a role in the coupling of bovine serum albumin to sheep erythrocytes are examined. A standard procedure was deduced by which other proteins were coupled and tested with their specific antisera. The observed titres are comparable to those obtained with the classical bis-diazotized benzidine method.

The technique is specific and practicable. It provides sensitized cells that remain stable for several weeks.

PubMed Disclaimer

References

    1. J Immunol. 1966 Dec;97(6):791-6 - PubMed
    1. Proc Soc Exp Biol Med. 1967 Apr;124(4):1166-70 - PubMed
    1. J Exp Med. 1951 Feb;93(2):107-20 - PubMed
    1. Ann Inst Pasteur (Paris). 1953 May;84(5):903-23 - PubMed
    1. Biochem J. 1957 Feb;65(2):273-7 - PubMed

LinkOut - more resources