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. 1969 Apr;17(4):504-6.
doi: 10.1128/am.17.4.504-506.1969.

Purification and concentration of influenza types A and B by chromatography on calcium phosphate

M Lapidus

Purification and concentration of influenza types A and B by chromatography on calcium phosphate

M Lapidus. Appl Microbiol. 1969 Apr.

Abstract

A rapid chromatographic method for the isolation of types A and B influenza virus from allantoic fluid was described. The adsorbent was prepared from calcium dihydrogen orthophosphate monohydrate [Ca(H(2)PO(4))(2).H(2)O] by alkali treatment. The addition of sodium trimetaphosphate to the influenza-infected allantoic fluid afforded a 67 to 100% viral recovery and a 26 to 43-fold increase in purity.

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