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. 1969 Mar;22(2):222-5.
doi: 10.1136/jcp.22.2.222.

Ultramicrofluorimetric determination of calcium in plasma

M R Lewin, M R Wills, D N Baron

Ultramicrofluorimetric determination of calcium in plasma

M R Lewin et al. J Clin Pathol. 1969 Mar.

Abstract

A precise and reproducible method is described for the ultramicro fluorimetric determination of calcium in plasma, based upon the formation of a fluorescent complex between calcium and calcein at a strongly alkaline pH. In a group of normal subjects, the mean concentration of plasma calcium was 9.66 mg/100 ml with a normal range of 8.9 to 10.4 mg/100 ml.

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