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. 1976 Apr 14;427(2):644-51.
doi: 10.1016/0005-2795(76)90208-7.

Binding of 17beta-estradiol by variants of alpha-fetoprotein in rat amniotic fluid

M S Soloff, S K Swartz, A F Pearlmutter, K Kithier

Binding of 17beta-estradiol by variants of alpha-fetoprotein in rat amniotic fluid

M S Soloff et al. Biochim Biophys Acta. .

Abstract

Two variants of alpha-fetoprotein in rat amniotic fluid were separated by their different affinity for concanavalin A-Sepharose, which selectively binds alpha-D-manno-pyranosides and alpha-D-glucopyranosides. Both forms had the same mobility upon polyacrylamide gel electrophoresis. The binding of 17beta-estradiol per mg of alpha-fetoprotein, determined both immunologically and electrophoretically, was the same for both variants. These results indicate that a specific carbohydrate portion of the molecule is not necessary for steroid binding.

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