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. 1969 Mar;9(3):320-9.
doi: 10.1016/S0006-3495(69)86389-7.

Studies of Raman spectra of water solutions of adenosine tri-, di-, and monophosphate and some related compounds

Studies of Raman spectra of water solutions of adenosine tri-, di-, and monophosphate and some related compounds

L Rimai et al. Biophys J. 1969 Mar.

Abstract

Using a He-Ne CW laser source together with a digital photon counting system, we have obtained well resolved Raman spectra for adenosine mono-, di-, and triphosphate (AMP, ADP, ATP) in aqueous solution. Spectra of these compounds were studied as a function of pH from pH = 0.5 to 13.5 and between 550 and 1700 cm(-1). It was found possible to distinguish spectroscopically between the three phosphates over the pH range studied. A qualitative analysis of vibrational modes responsible for various spectral lines is given. Lines at about 960 and 1100 cm(-1) were found to be good indications of the degree of ionization of the terminal phosphate group.

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References

    1. Biochim Biophys Acta. 1966 Jul 13;120(3):461-5 - PubMed
    1. J Biol Chem. 1968 Apr 10;243(7):1337-43 - PubMed

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