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. 1969 Mar;111(5):689-94.
doi: 10.1042/bj1110689.

A peptide containing a reactive lysyl group from ox liver glutamate dehydrogenase

J J Holbrook, R Jeckel

A peptide containing a reactive lysyl group from ox liver glutamate dehydrogenase

J J Holbrook et al. Biochem J. 1969 Mar.

Abstract

1. Inhibition of ox liver glutamate dehydrogenase with N-(N'-acetyl-4[(35)S]-sulphamoylphenyl)maleimide (ASPM) is more specific at pH7.3 than at pH6.9. At pH7.3 inhibition accompanies the incorporation at 1 mole of ASPM residues into about 53000g. of protein. 2. Digestion of the modified protein with chymotrypsin and trypsin yields a unique radioactive peptide. 3. Acid hydrolysis of 1 mole of this peptide yields 1 mole of N(in)-succin-2-yl-lysine. The in-amino group of a lysyl residue is thus the site of modification of the protein. 4. The sequence containing the modified lysyl residue is: [Formula: see text] where Asx respresents either aspartic acid or asparagine.

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