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. 1969 Jul;18(1):64-7.
doi: 10.1128/am.18.1.64-67.1969.

L-asparaginase production by Erwinia aroideae

R E Peterson, A Ciegler

L-asparaginase production by Erwinia aroideae

R E Peterson et al. Appl Microbiol. 1969 Jul.

Abstract

Maximum yields of 1,250 IU (international unit)/g (dry weight of cells) of L-asparaginase were obtained in 8 hr from Erwinia aroideae NRRL B-138. Partial purification and concentration of the extracted L-asparaginase yielded a preparation with an activity of 275 IU/ml. Only one L-asparaginase was present as determined by electrophoresis, and the enzyme exhibited a pH optimum of 7.5 and a K(m) of 3 x 10(-3) M.

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References

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