Inhibition of infectious Rous sarcoma virus production by rifamycin derivative
- PMID: 58073
- PMCID: PMC515569
- DOI: 10.1128/JVI.18.2.445-453.1976
Inhibition of infectious Rous sarcoma virus production by rifamycin derivative
Abstract
A new rifamycin derivative, rifazone-82 (R-82), an inhibitor of viral RNA-dependent DNA polymerase, is selectively toxic to transformed chicken cells in culture. R-82 has now been shown to possess antiviral activity as well. The relatively nontoxic properties of R-82 to nontransformed cells have permitted the execution of experiments examining the effect of a rifamycin derivative on virus reproduction. Addition of low concentrations of R-82 (15 mug/ml) to cultures soon after Rous sarcoma virus infection prevents the spread of infection thoroughout the culture. This inhibition is not dependent on concomitant cellular transformation as identical results were obtained with cells infected with a transformation-defective Rous sarcoma virus. Addition of R-82 to cultures in which all the cells are infected does not substantially affect the yield of physical particles as measured by RNA-dependent DNA polymerase activity and by (3H) uridine incorporation into viral RNA. However, the infectivity of the progeny virus, as measured by focus-forming ability, is decrreased 95 to 99% by R-82 treatment.
Similar articles
-
Antiviral action of a rifamycin derivative: formation Rous sarcoma virus particles deficient in 60 to 70S RNA.J Virol. 1978 Mar;25(3):944-7. doi: 10.1128/JVI.25.3.944-947.1978. J Virol. 1978. PMID: 205684 Free PMC article.
-
Preferential inhibition of the growth of virus-transformed cells in culture by rifazone-82, a new rifamycin derivative.Proc Natl Acad Sci U S A. 1974 Jun;71(6):2520-4. doi: 10.1073/pnas.71.6.2520. Proc Natl Acad Sci U S A. 1974. PMID: 4366963 Free PMC article.
-
Oxime derivatives of erythromycin: inhibitors of Rous sarcoma virus reverse transcriptase activity and focus formation.J Gen Virol. 1975 Jan;26(1):135-9. doi: 10.1099/0022-1317-26-1-135. J Gen Virol. 1975. PMID: 47382
-
Effect of two rifamycin derivatives on the Rous sarcoma virus transformation system.J Gen Virol. 1972 Nov;17(2):221-5. doi: 10.1099/0022-1317-17-2-221. J Gen Virol. 1972. PMID: 4344314 No abstract available.
-
Assay of noninfectious fragments of DNA of avian leukosis virus-infected cells by marker rescue.J Virol. 1977 May;22(2):300-7. doi: 10.1128/JVI.22.2.300-307.1977. J Virol. 1977. PMID: 68125 Free PMC article.
Cited by
-
Antiviral action of a rifamycin derivative: formation Rous sarcoma virus particles deficient in 60 to 70S RNA.J Virol. 1978 Mar;25(3):944-7. doi: 10.1128/JVI.25.3.944-947.1978. J Virol. 1978. PMID: 205684 Free PMC article.
-
Rifapentine is an entry and replication inhibitor against yellow fever virus both in vitro and in vivo.Emerg Microbes Infect. 2022 Dec;11(1):873-884. doi: 10.1080/22221751.2022.2049983. Emerg Microbes Infect. 2022. PMID: 35249454 Free PMC article.
-
Primary avian tendon cells in culture. An improved system for understanding malignant transformation.J Cell Biol. 1978 Dec;79(3):672-9. doi: 10.1083/jcb.79.3.672. J Cell Biol. 1978. PMID: 215595 Free PMC article.