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. 1969 Aug;114(1):71-3.
doi: 10.1042/bj1140071.

The reversibility of the delta8-cholestenol-delta7-cholestenol isomerase reaction in cholesterol biosynthesis

D C Wilton, A D Rahimtula, M Akhtar

The reversibility of the delta8-cholestenol-delta7-cholestenol isomerase reaction in cholesterol biosynthesis

D C Wilton et al. Biochem J. 1969 Aug.

Abstract

The incubation of Delta(7)-cholestenol with a 10000g supernatant or 105000g microsomes in the presence of tritiated water is studied. The reisolated Delta(7)-cholestenol contained up to 0.67g.atom of tritium/mole. This result can best be explained by assuming the reversibility of the reaction Delta(8)-cholestenol right harpoon over left harpoon Delta(7)-cholestenol.

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References

    1. Biochem J. 1968 Feb;106(4):803-10 - PubMed