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. 1969 Jun 13;164(3885):1292-3.
doi: 10.1126/science.164.3885.1292.

Human oocytes: maturation in chemically defined media

J F Kennedy, R P Donahue

Human oocytes: maturation in chemically defined media

J F Kennedy et al. Science. .

Abstract

Human oocytes from ovarian follicles resume meiosis in F10, a defined medium, in numbers comparable to that obtained in medium containing serum. Several simple Krebs-Ringer media also support maturation, which suggests a similarity of nutritional requirements between human and mouse oocytes. Fewer oocytes reach metaphase II when the investing follicular cells are removed prior to culture.

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