Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1969 Aug;114(1):11-7.
doi: 10.1042/bj1140011.

The specificity of purified porcine pancreatic elastase

A S Narayanan, R A Anwar

The specificity of purified porcine pancreatic elastase

A S Narayanan et al. Biochem J. 1969 Aug.

Abstract

An electrophoretically homogeneous elastase preparation free from tryptic and chymotryptic activities was obtained by chromatography on DEAE-Sephadex and CM-cellulose. This preparation exhibits a narrower specificity towards peptide bonds than that observed by Naughton & Sanger (1961). With oxidized insulin B chain as substrate, the fastest breaks occur between alanine-14 and leucine-15 and between valine-18 and cysteic acid-19. The bond between glycine-23 and phenylalanine-24 is also efficiently hydrolysed. Other bonds hydrolysed are that between valine-12 and glutamic acid-13 and that between serine-9 and histidine-10. Oxidized insulin A chain is hydrolysed only at one of two points, between alanine-8 and serine-9 or between serine-12 and leucine-13, and the rate of hydrolysis is very low.

PubMed Disclaimer

References

    1. Can J Biochem. 1965 Dec;43(12):1961-70 - PubMed
    1. J Biol Chem. 1967 May 25;242(10):2522-7 - PubMed
    1. Biochem Biophys Res Commun. 1966 Sep 8;24(5):593-7 - PubMed
    1. Biochem J. 1966 Oct;101(1):232-41 - PubMed
    1. Biochem J. 1967 May;103(2):497-507 - PubMed