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. 1965 Oct;10(2):227-40.
doi: 10.1530/jrf.0.0100227.

Studies on the development of mouse embryos in vitro. IV. Interaction of energy sources

R L Brinster

Studies on the development of mouse embryos in vitro. IV. Interaction of energy sources

R L Brinster. J Reprod Fertil. 1965 Oct.

Abstract

The interactions of a number of possible energy sources for in-vitro development of 2-cell mouse ova were examined using statistical experimental designs. These experiments indicated that glucose has no beneficial effect on development when employed with the optimum concentration of pyruvate. Optimum concentrations of pyruvate and oxaloacetate when employed together resulted in a significantly lower response than when either compound was employed alone. It was found that the best medium for the development of 2-cell mouse ova into blastocysts contained 2·5 to 5·0 × 10−4 M-pyruvate + 2·5 to 5·0 × 10−2 M-lactate.

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Figures

Fig 1
Fig 1
Interaction of pyruvate and lactate. Pyruvate concentration: ■, 5·0 × 10−4 M; ▲, 2·5 × 10−4 M; ●, 0.
See this image and copyright information in PMC

References

    1. Biggers JD, Brinster RL. Biometrical problems in the study of early mammalian embryos in vitro. J exp Zool. 1965;158:39. - PubMed
    1. Bishop D. Metabolic conditions within the oviduct of the rabbit. Int J Fertil. 1957;2:11.
    1. Brinster RL. A method for in vitro cultivation of mouse ova from two-cell to blastocyst. Exp Cell Res. 1963;32:205. - PMC - PubMed
    1. Brinster RL. PhD thesis. University of Pennsylvania; 1964. Studies on the development of mouse embryos in vitro.
    1. Brinster RL. Studies on the development of mouse embryos in vitroI The effect of osmolarity and hydrogen ion concentration. J exp Zool. 1965a;158:49. - PubMed

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