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. 1965 Oct 29;150(3696):618-9.
doi: 10.1126/science.150.3696.618.

Sperm capacitation by uterine fluid or beta-amylase in vitro

K T Kirton, H D Hafs

Sperm capacitation by uterine fluid or beta-amylase in vitro

K T Kirton et al. Science. .

Abstract

Rabbit sperm developed the capacity to fertilize ova when incubated in utero or in vitro in fluids from estrous uteri. Incubation of sperm with beta-amylase in phosphate-buffered Locke's solution also resulted in capacitation. The data suggest that sperm capacitation involves enzymatic alteration of carbo-hydrate-containing seminal macromolecules which coat sperm and inhibit fertilization.

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