doi: 10.1073/pnas.73.5.1476.
Human pituitary growth hormone: restoration of full biological activity by noncovalent interaction of two fragments of the hormone
- PMID: 58417
- PMCID: PMC430319
- DOI: 10.1073/pnas.73.5.1476
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Human pituitary growth hormone: restoration of full biological activity by noncovalent interaction of two fragments of the hormone
Proc Natl Acad Sci U S A.
1976 May.
Abstract
The NH2-terminal 134 amino-acid fragment of the reduced-carbamidomethylated human somatropin molecule is found to react noncovalently with the COOH-terminal 51 amino-acid fragment in solutions of pH 8.4 at 2 degrees to restore full biological activity as evidenced by the rat tibia and pigeon crop-sac assays. In addition, circular dichroism spectra of the recombinant show the conformation to be completely repaired in comparison with that of the native hormone.
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