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. 1976 May;73(5):1476-9.
doi: 10.1073/pnas.73.5.1476.

Human pituitary growth hormone: restoration of full biological activity by noncovalent interaction of two fragments of the hormone

C H Li, T A Bewley

Human pituitary growth hormone: restoration of full biological activity by noncovalent interaction of two fragments of the hormone

C H Li et al. Proc Natl Acad Sci U S A. 1976 May.

Abstract

The NH2-terminal 134 amino-acid fragment of the reduced-carbamidomethylated human somatropin molecule is found to react noncovalently with the COOH-terminal 51 amino-acid fragment in solutions of pH 8.4 at 2 degrees to restore full biological activity as evidenced by the rat tibia and pigeon crop-sac assays. In addition, circular dichroism spectra of the recombinant show the conformation to be completely repaired in comparison with that of the native hormone.

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References

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