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. 1977 Nov;36(5):608-17.
doi: 10.1038/bjc.1977.238.

Development and application of a radioimmunoassay for methotrexate

G W Aherne, E M Piall, V Marks
Free PMC article

Development and application of a radioimmunoassay for methotrexate

G W Aherne et al. Br J Cancer. 1977 Nov.
Free PMC article

Abstract

An antiserum to methotrexate has been produced in a sheep against a conjugate of ovalbumin and methotrexate (MTX) prepared using a water-soluble carbodiimide. The antibodies produced were specific for substances containing the 2,4-diamino pteridine structure. Naturally occurring folates did not interfere with the assay. A radioimmunoassay has been developed using this antiserum, which can be used to measure MTX concentrations of less than 1 ng/ml in biological samples without prior extraction. The concentrations of MTX in the blood and urine of patients following a single i.v. bolus injection and following oral administration of the drug have been measured. The published radioimmunoassays for MTX have been compared.

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