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. 1965 Dec;27(3):451-7.
doi: 10.1083/jcb.27.3.451.

Nuclear DNA and cytoplasmic DNA from tissues of higher plants

Y Hotta, A Bassel, H Stern

Nuclear DNA and cytoplasmic DNA from tissues of higher plants

Y Hotta et al. J Cell Biol. 1965 Dec.

Abstract

Young wheat roots were labeled with (32)P-inorganic phosphate. Following the labeling period, roots were homogenized in a sucrose medium and fractionated into nuclei, cytoplasmic particles (including proplastids and mitochondria), and a soluble fraction containing most of the microsomes. DNA prepared from the particles had a higher buoyant density than that from the nuclei and showed a marked loss in total label if the roots were exposed to non-radioactive medium for 48 hours prior to fractionation of the cells.

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