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. 1976;11(2):107-16.
doi: 10.1016/0022-1759(76)90138-1.

In vitro induction and measurement of hemolytic plaque forming cells in man

H M Dosch, E W Gelfand

In vitro induction and measurement of hemolytic plaque forming cells in man

H M Dosch et al. J Immunol Methods. 1976.

Abstract

Following co-cultivation with sheep red cells or ovalbumin, Hypaque-Ficoll-separated human tonsillar lymphocytes were demonstrated to generate specific hemolytic PFC with maximum numbers at day 5-7. PFC were enumerated on poly-L-lysine coupled red cell monolayers in Microtest-II-plates. Plaque formation appeared to be puromycin-sensitive, complement-dependent and showed clear specificity for the antigen present during the inductive culture. Treatment of PFC with mu-chain specific antisera and complement resulted in complete inactivation of PFC; gamma-chain antisera had no effect. The development of such a simple and sensitive assay system permits the analysis of cellular interactions required for the induction of PFC responses in man.

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