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. 1966 Jan;7(1):170-4.

Quantitative micro determination and isolation of plasmalogen aldehydes as 2,4-dinitrophenylhydrazones

I Katz, M Keeney
  • PMID: 5900215
Free article

Quantitative micro determination and isolation of plasmalogen aldehydes as 2,4-dinitrophenylhydrazones

I Katz et al. J Lipid Res. 1966 Jan.
Free article

Abstract

A micro spectrophotometric procedure for the quantitative determination of plasmalogen aldehydes is described which utilizes simultaneous methanolysis and formation of 2,4-dinitrophenylhydrazones. After isolation of the hydrazones by thin-layer chromatography, the aldehydes can be regenerated, reduced, acetylated, and then analyzed by gas-liquid chromatography. Identification of the plasmalogen aldehydes obtained from rumen holotrich protozoa is described.

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