Movement of indoleacetic acid in coleoptiles of Avena sativa L. II. Suspension of polarity by total inhibition of the basipetal transport

Abstract

Acropetal and basipetal movement of indole-3-acetic acid through coleoptiles of Avena sativa L. was studied. Sections 10-mm long were supplied with either apical or basal sources containing C(14) carboxyl-labeled indoleacetic acid (10(-5)m). Anaerobic conditions inhibit metabolically dependent movement (transport) thus reducing basipetal but not acropetal movement. Total inhibition of basipetal transport abolishes the polarity of auxin uptake and movement. The nonpolar movement that remains in anaerobic sections is free diffusion with an average diffusion coefficient of approximately 1 x 10(-4) mm(2) per second. During an 8-hour diffusion, at least the first millimeter of the section comes to equilibrium at approximately the same concentration as the donor.Acropetal movement is probably by diffusion and is accompanied by an aerobic immobilization of indoleacetic acid that increases more than proportionally to concentration. Anaerobic conditions totally prevent this immobilization and reduce acropetal uptake but not the amount of indoleacetic acid moving into the upper parts of the section; there is, therefore, no evidence for acropetal transport. Polarity of auxin movement in aerobic coleoptile sections is achieved by strict basipetal transport of auxin. The basipetal transport may intensify the polarity by recycling auxin that is moving acropetally.