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. 1977 Jul;1(5-6):323-9.
doi: 10.1016/0378-1119(77)90037-3.

A new sequence-specific endonuclease (Bsp) from Bacillus sphaericus

A Kiss, B Sain, E Csordás-Tòth, P Venetianer

A new sequence-specific endonuclease (Bsp) from Bacillus sphaericus

A Kiss et al. Gene. 1977 Jul.

Abstract

A new restriction endonuclease has been isolated from Bacillus sphaericus R. The purification procedure includes Bio-Gel filtration, (NH4)2SO4 fractionation and phosphocellulose chromatography. After the phosphocellulose step the enzyme preparation is free of non-specific nucleases. Bsp cleaves double-stranded DNA with the same specificity as Bacillus subtilis (Bsu) and Haemophilus aegyptius (HaeIII) restriction endonucleases, as concluded from digests and double-digests of phiX174 replicative form DNA with Bsu and Bsp. The 5'-terminal nucleotide of the cleavage products was shown to be C. Bacillus sphaericus R produces Bsp in extremely large quantities and the enzyme can be easily purified in high yield.

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