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. 1966 Mar;98(3):909-14.
doi: 10.1042/bj0980909.

Camel myoglobin

E S Awad, L Kotite

Camel myoglobin

E S Awad et al. Biochem J. 1966 Mar.

Abstract

1. Crystalline myoglobin was prepared from camel heart muscle. 2. A method was developed for the isolation of myoglobin that employs molecular-sieve chromatography. 3. Analytical chromatography of the camel myoglobin on a molecular-sieve column and on two types of ion-exchange columns gave in each case a single elution band, which accounted for better than 98% recovery and showed that the product was free from haemoglobin. 4. The iron content on a dry weight basis was 0.308%. This value corresponds to a molecular weight of 18100. 5. The spectra of acidic ferrimyoglobin, basic ferrimyoglobin and ferrimyoglobin cyanide were measured. 6. The pK(a) of the dissociation of the haem-bound water molecule in acidic ferrimyoglobin was 8.53 at 25 degrees . 7. Conclusions are drawn about the charge on the surface of the camel ferrimyoglobin molecule as compared with horse and sperm-whale ferrimyoglobins.

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References

    1. Nature. 1959 Apr 4;183(4666):941-3 - PubMed
    1. Biochem J. 1952 Nov;52(3):517-23 - PubMed
    1. Nature. 1963 Jun 22;198:1201-2 - PubMed
    1. Science. 1948 Jul 2;108(2792):15-6 - PubMed
    1. Arch Biochem Biophys. 1954 Jun;50(2):496-502 - PubMed