Three methods of assessing the mutagenic action of ultraviolet radiation on the fungus Emericellopsis glabra

Abstract

Colony isolates of the fungus Emericellopsis glabra, developing from ultraviolet (UV)-irradiated conidia, were examined for variability on the basis of colony morphology, loss of antibiotic-producing capacity on plate assay, and loss of ability to grow in minimal medium. The latter two of these methods are considered to be more objective and reliable in determining the degree of variability induced by UV radiation in this fungus. The highest frequency of variants is obtained at UV dosages which reduce spore survival to levels of 5 to 15%.