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. 1977 Nov;82(5):1495-8.
doi: 10.1093/oxfordjournals.jbchem.a131840.

New fluorogenic substrates for alpha-thrombin, factor Xa, kallikreins, and urokinase

T Morita, H Kato, S Iwanaga, K Takada, T Kimura
Free article

New fluorogenic substrates for alpha-thrombin, factor Xa, kallikreins, and urokinase

T Morita et al. J Biochem. 1977 Nov.
Free article

Abstract

Twenty peptide-4-methylcoumarin amides (MCA) were newly synthesized and tested as possible substrates for alpha-thrombin, factor Xa, kallikreins, urokinase, and plasmin. These fluorogenic peptides contained arginine-MCA as the carboxyl-terminus. Release of 7-amino-4-methylcoumarin was determined fluorometrically. Of these peptides, the following were found to be specific substrates for individual enzymes: Boc-Val-Pro-Arg-MCA for alpha-thrombin, Boc-Ile-Glu-Gly-Arg-MCA, and Boc-Ser-Gly-Arg-MCA for factor Xa, Z-Phe-Arg-MCA for plasma kallikrein, Pro-Phe-Arg-MCA for pancreatic and urinary kallikreins, and glutaryl-Gly-Arg-MCA for urokinase. Moreover, these peptide-MCA substrates were resistant to plasmin.

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