Isolation of nuclei from a marine dinoflagellate

Abstract

By means of a medium containing dextran, nuclei were isolated in high yield from cells of Gymnodinium nelsoni, a marine dinoflagellate. Most of the DNA, but less than onetenth of the RNA, of the original cells was recovered in the purified nuclei. The nuclei appeared substantially intact as observed by light or electron microscopy. The isolated nuclei were capable of incorporating tritiated uridine triphosphate into material insoluble in cold acid. The general procedure was found to be applicable also to two species of diatoms.