Electrophoretic variation of galactose-1-phosphate uridyltransferase

Abstract

A specific method for starch-gel electrophoresis of galactose-1-phosphate uridyltransferase has been developed. Electrophoresis of red-cell hemolyzate from normal subjects and subjects homozygous for the Duarte variant has shown that the Duarte variant has a slightly faster electrophoretic mobility than the normal enzyme under the various conditions used. Molecular-weight estimation on Sephadex G-200 indicates that this observed difference in electrophoreticmobility of the Duarte variant is not due to difference in molecular size. Both enzymes have a molecular weigh of approximately 85,000.