Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1966 Nov 1;124(5):833-49.
doi: 10.1084/jem.124.5.833.

Mediators of inflammation in leukocyte lysosomes. VI. Partial purification and characterization of a mast cell-rupturing component

W Seegers, A Janoff

Mediators of inflammation in leukocyte lysosomes. VI. Partial purification and characterization of a mast cell-rupturing component

W Seegers et al. J Exp Med. .

Abstract

The mast cell-rupturing component present in the lysosomes of rabbit exudate PMN neutrophil leukocytes has been identified and some of its physical and chemical properties have been described. The active agent is a low molecular weight (1200 to 2400) polypeptide containing a relatively large proportion of the basic amino acid, arginine. It is thermostable and dialyzable, and does not cause contraction of the isolated guinea pig ileum. The mast cell-rupturing activity of the agent is destroyed by trypsin. A second permeability factor with a larger molecular weight is present in crude extracts of PMN granules. Although this substance does not lyse mast cells, it is capable of evoking delayed permeability responses in rabbit skin.

PubMed Disclaimer

References

    1. Life Sci. 1964 Sep;3:1025-32 - PubMed
    1. Am J Hum Genet. 1962 Mar;14:14-21 - PubMed
    1. Lab Invest. 1964 Jan;13:1-15 - PubMed
    1. Life Sci. 1965 Dec;4(24):2361-72 - PubMed
    1. Proc Soc Exp Biol Med. 1964 Feb;115:273-6 - PubMed