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. 1977 Dec;12(12):1078-80.
doi: 10.1007/BF02533337.

A simplified colorimetric micromethod for determination of serum cholesterol

R R Bhandaru, S R Srinivasan, P S Pargaonkar, G S Berenson

A simplified colorimetric micromethod for determination of serum cholesterol

R R Bhandaru et al. Lipids. 1977 Dec.

Abstract

A simple and rapid micromethod is described for quantitation of cholesterol in 5-10 microliter of serum using uranyl acetate to deproteinize the serum and o-phthalaldehyde reagent to develop color. Independent analyses of serum samples by the present method and by two reference methods, Abell-Kendall and automated colorimetric method, indicated accuracy of the micromethod (r = 0.99). Use of capillary blood specimens makes this procedure applicable for screening infants and small children.

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References

    1. J Biol Chem. 1952 Mar;195(1):357-66 - PubMed
    1. J Lipid Res. 1973 May;14(3):364-6 - PubMed
    1. Clin Chem. 1975 Sep;21(10):1526-30 - PubMed
    1. Clin Chim Acta. 1974 May 31;53(1):43-9 - PubMed
    1. Anal Biochem. 1969 Apr 11;29(1):143-8 - PubMed

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