Studies on cells producing haemolytic antibodies against sheep red blood cells in short-term experiments in vitro

Abstract

A system is described for the maintenance of isolated cells from mouse spleens during short periods of time in vitro. Active γM antibody synthesis continues throughout the experimental period. The number of antibody-producing cells remains unchanged when the synthesis of DNA is inhibited. The effects of puromycin, parafluorophenylalanine, actinomycin D and hydrocortisone have also been tested and their dose-dependent effects established. γM antibody synthesis is shown to follow the same pattern as mammalian protein synthesis in general.