Comparative Study
doi: 10.1093/nar/4.11.3787.
Determination of the sequence homology between the four RNA species of cucumber mosaic virus by hybridization analysis with complementary DNA
- PMID: 593886
- PMCID: PMC343200
- DOI: 10.1093/nar/4.11.3787
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Comparative Study
Determination of the sequence homology between the four RNA species of cucumber mosaic virus by hybridization analysis with complementary DNA
Nucleic Acids Res.
1977 Nov.
Free PMC article
Abstract
The method of Taylor etal., (11) has been used to transcribe complementary DNA probes from the four major RNA species of cucumber mosaic virus (RNAs 1 - 4 in order of decreasing molecular weight). Analysis of the kinetics of hybridization of these probes in homologous and heterologous complementary DNA-RNA hybridization reactions has shown that the sequence of the smallest RNA (RNA 4), which contains the coat protein gene, is present within RNA 3. RNAs 1 and 2 are unique RNA molecules while each has a region of approximately 300 nucleotides in common with RNA 4.Images
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